Trypsinization of Animal Tissues for Cell Culture: Theoretical Considerations and Automatic Apparatus.
نویسنده
چکیده
The theoretical background and the experimental feasibility of an automatic control for the continuous trypsinization process are discussed. An automatic apparatus is described with experimental evidence that the optimal mean residence time of monkey kidney cells in the trypsinization flask is between 5 and 8 min, in a volume of fluid approximately ten times that of the tissue processed. A temperature of 36 C and a pH of 7.8 provide optimal conditions for cell viability.
منابع مشابه
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ورودعنوان ژورنال:
- Applied microbiology
دوره 12 شماره
صفحات -
تاریخ انتشار 1964